数据库

实验试剂 抗凝保存液中保存的绵羊红细胞、马抗绵羊红细胞抗血清(溶血素)、pH值7.4巴比妥缓冲液、聚合IgG、0.04%氨溶液。 实验设备 12×8孔的U型微量板、稀释器(50μl)、分液器、离心机(MSE 6 L，可使U型微量板离心)、72型分光光度计等。 实验步骤 1. 致敏绵羊红细胞的制备 1) 将5ml抗凝保存液中的红细胞2 500 r/min离心5m ...

实验步骤 1. Loading Single Comb and Double Comb Gels1) Plug the E-Gel® PowerBase™ v.4 into an electrical outlet using the adaptor plug.2) Open the package containing the gel and inse ...

实验原理 乳酸脱氢酶（lactate dehydrogenase，LDH）普遍存在于全身各组织细胞，尤以肌肉细胞为甚。在有辅酶Ⅰ存在时，乳酸脱氢酶可使乳酸脱氢转变成丙酮酸，其氢可经由辅酶Ⅰ、黄素酶传递给甲烯蓝，使之转变为甲烯白。由此产生的颜色变化即可认证乳酸脱氢酶的上述催化作用。乳酸脱氢酶催化乳酸与丙酮酸之间的可逆反应，本实验采用KCN与丙酮酸反应生成丙酮酸氰醇而取走反应体系中的丙酮酸，于是反应 ...

实验原理 One of the most pressing problems facing modern medical researchers is the surging levels of obesity with the consequent increase in associated disorders such as diabetes and cardiovascular dise ...

实验试剂 cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment so it needs a separate room with the air purification system and a UV lamp to sterilize the entire roo ...

实验试剂 Regents to be provided by user:1. Absolute (96%-100%) ethanol 2. Isopropanol 实验设备 Equipments to be provided by user:1. Centrifuge capable of 4000 x g with swinging-bucket rotor for 96 well ...

实验步骤 1.Inoculate 200 ml of L2 containing appropriate selective agent with a single colony or culture containing the desired plasmid. Grow at 37 degrees overnight.2.Spin down cells at 5000 rpm for 1 ...

实验试剂 Buffers and Solutions Alkaline lysis solution I For preparations of plasmid DNA that are to be subjected to further purification by chromatography sterile Alkaline lysis solution I ma ...

实验材料 金花和丰产两种梨树的离体叶片。 实验步骤 1. 培养基的配制试验所用的培养基为MS (Murashige & Skoog 1962) NN69 (Chu et al. 1975) C (Pinet-Leblay et al. 1992) LE (Quoirin & Lepoivre 1977)等，均为固体（有特殊说明者除外）。各种培养基均参照参考文献记述的方法配制。2. ...

实验材料 Kit ContentsDiIC1(5) 625 μL of 10 μM DiIC1(5) in DMSOCCCP 125 μL of 50 mM CCCP in DMSOStorage and HandlingUpon receipt components should be stored at 2–6°C. DO NOT FREEZE. Before openin ...

实验原理 H2O2与硫酸钛（或氯化钛）生成过氧化物—钛复合物黄色沉淀，可被H２SO4溶解后，在415nm波长下比色测定。在一定范围内，其颜色深浅与H2O2浓度呈线性关系。 实验试剂 100μmol/L H2O2丙酮试剂：取30%分析纯H2O2 57μl，溶于100ml，再稀释100倍；2mol/L硫酸；5%（W/V）硫酸钛；丙酮；浓氨水。 实验设备 研钵；移液管0 ...

实验试剂 1. Equilibrate sterile dH2O water or 10 mM Tris pH 9.0 at 65℃.2. Isopropyl alcohol (isopropanol; absolute)3. Absolute (96%-100%) ethanol4. RNase A stock solution at 20 mg/mL 5. Liquid nitrogen f ...

实验原理 The E.Z.N.A.® MicroElute Genomic DNA Kit uses the reversible binding properties of the HiBind® matrix a new silica-based material combined with the MicroElute column spin technology which allows ...

实验试剂 1. 80% Ethanol2. Isopropanol 3. Chloroform 实验设备 1. Magnetic Stand for 1.5 ml tube (OBI # MSD-02)2. Nuclease-free 1.5 and 2 ml centrifuge tubes 3. Microcentrifuge capable of 12000 x g and 2- ...

实验试剂 1. Absolute (96%-100%) ethanol 2. Molecular biology grade water 实验设备 1. Multiple-channel pipettor2. 96-well microplate 3. Magnetic Separation Devices 实验步骤 1. Determine the volume of ...

实验试剂 Regents supplied by user1. Sterile Deionized Water (or TE Buffer)2. 70% Ethanol 3. 96%-100% Isopropanol 实验设备 Equipments supplied by user1. Microcentrifuge capable of at least 18000 x g (a c ...

实验设备 Water bath at 37°CLaminar flow hoodPipet-aid pipettes and micropipettesMultichannel pipettes and repeater pipettePolystyrene round-bottom tubes (40mL) and petri dishes (92 x 17 mm) or similar co ...

实验试剂 1. 2.55 M sucrose (unbuffered) 1)Heat on a stirring block to dissolve then bring up to 1 L. 2)Determine concentration using a refractometer and adjust to 2.55 M sucrose (refractive in ...

实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples for direct use in reverse transcription PCR. The direct mRNA isolation is comp ...

实验试剂 柱材Oliga(dT）－纤维素上样液：20mM Tris-HCL(pH 7.6) 0.5M NaCL 1mM EDTA (pH 8.0) 0.1% 十二烷基肌氨酸钠（SLS）洗脱液：10mM Tris-HCL(pH 7.6) 1mM EDTA (pH 8.0) 0.05% SDS ...