数据库

Abstract Table of Contents Materials Figures Literature Cited Abstract Deep sequencing analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively

Abstract Table of Contents Materials Figures Literature Cited Abstract Mitochondrial disorders are a group of complex and heterogeneous diseases that may be caused by molecular defects in the nuclear or m

Abstract Table of Contents Figures Literature Cited Abstract Genome?wide association studies (GWAS) are being conducted at an unprecedented rate in population?based cohorts and have increased our understanding of the pathophysiology of com

Abstract Table of Contents Materials Figures Literature Cited Abstract Biological databases are an important resource for the life sciences community. Accessing the hundreds of databases supporting molecu

Abstract Table of Contents Figures Literature Cited Abstract A Modular Open?Source Assembler (AMOS) was designed to offer a modular approach to genome assembly. AMOS includes a wide range of tools for assembly, including the lightweight de

Abstract Table of Contents Figures Literature Cited Abstract GBrowse is widely used by biologists to visualize genome annotation. We have developed WebGBrowse to facilitate hosting multiple GBrowse instances specific for different users on

Abstract Table of Contents Figures Literature Cited Abstract Inferring a protein's function by homology is a powerful tool for biologists. The Princeton Protein Orthology Database (P?POD) offers a simple way to visualize and analyze the re

Abstract Table of Contents Figures Literature Cited Abstract Chromatin immunoprecipitation (ChIP) coupled with genome tiling array hybridization (ChIP?chip) and ChIP followed by massively parallel sequencing (ChIP?seq) are high?throughput

Abstract Table of Contents Materials Figures Literature Cited Abstract The yeast two?hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is perfor

Abstract Table of Contents Materials Figures Literature Cited Abstract The Thermofluor assay, also referred to as a thermal shift assay or differential scanning fluorescence (DSF), is a fast and simple me

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit includes a basic protocol with an introduction to the Map Viewer, describing how to perform a simple text?based search of g

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit provides a basic protocol for oligo hybridization?based sequencing technology and resulting data analysis specific to the A

Abstract Table of Contents Figures Literature Cited Abstract Model?based Analysis of ChIP?Seq (MACS) is a command?line tool designed by X. Shirley Liu and colleagues to analyze data generated by ChIP?Seq experiments in eukaryotes, especial

Abstract Table of Contents Figures Literature Cited Abstract Biological databases play a central role in bioinformatics. They offer scientists the opportunity to access a wide variety of biologically relevant data, including the genomic se

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptidomics, the analysis of the peptide content of cells or tissues, can be used to study proteases in several ways. First, nearly a

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Abstract Table of Contents Figures Literature Cited Abstract OrthoMCL is an algorithm for grouping proteins into ortholog groups based on their sequence similarity. OrthoMCL?DB is a public database that allows users to browse and view orth

Abstract Table of Contents Figures Literature Cited Abstract This unit describes how to use the gene?finding programs GeneMark.hmm?E and GeneMark?ES for finding protein?coding genes in the genomic DNA of eukaryotic organisms. These bioinfo

Abstract Table of Contents Figures Literature Cited Supplementary Material Abstract This unit describes how to use several gene?finding programs from the GeneMark line developed for finding protein?

Abstract Table of Contents Figures Literature Cited Abstract Analysis of genomic data requires access to software tools that place the sequence?derived information in the context of biology. The Saccharomyces Genome Database (SGD) integrat