数据库

Abstract Table of Contents Materials Figures Literature Cited Abstract The ability to study proteins and protein interactions inside cells and tissues is important for elucidating how cells function in he

Abstract Table of Contents Materials Figures Literature Cited Abstract Nature possesses an unlimited number and source of biologically relevant natural glycans, many of which are too complicated to synthe

Abstract Table of Contents Figures Literature Cited Abstract Multidimensional ?3D? melting curves for G?quadruplexes are obtained by recording whole spectra (absorbance, CD, fluorescence) as a function of temperature, rather than the commo

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for separation of quadruplex species formed from the same sequence via size?exclusion chromatography (SE

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a system for expression of biotinylated proteins in mammalian cells in vivo, and its application to chromatin imm

Abstract Table of Contents Materials Figures Literature Cited Abstract Sites of protein binding to DNA are inferred from footprints or spans of protection against a probing reagent. In most protocols, sit

Abstract Table of Contents Materials Figures Literature Cited Abstract Distinguishing between crystals of protein?nucleic acid complexes and those containing protein alone is a common problem in structura

Abstract Table of Contents Materials Figures Literature Cited Abstract DNA methylation and demethylation significantly affect the deactivation and activation processes of gene expression, respectively. Th

Abstract Table of Contents Materials Figures Literature Cited Abstract ?Regulator of G?protein Signaling? (RGS) proteins constitute a class of intracellular signaling regulators that accelerate GTP hydrol

Abstract Table of Contents Materials Figures Literature Cited Abstract Chemically modified oligonucleotides play a significant role for genomic research. Modified nucleosides, such as with a fluorescent d

Abstract Table of Contents Materials Figures Literature Cited Abstract Oligonucleotides are functionalized by conjugation with a variety of molecules, and aliphatic amino linkers have been frequently used

Abstract Table of Contents Materials Figures Literature Cited Abstract Quantitative measurement of the levels of mRNA expression via real?time reverse transcription polymerase chain reaction (RT?PCR) has

Abstract Table of Contents Figures Literature Cited Abstract Determination of the oligomeric state of integral membrane proteins in detergent solutions is a challenging task because the amount of detergent associated with the protein is ty

Abstract Table of Contents Materials Figures Literature Cited Abstract In addition to X?ray, NMR, and FRET, electron paramagnetic resonance (EPR) can be applied to elucidate the structure of different mac

Abstract Table of Contents Materials Figures Literature Cited Abstract A simple method for the etherification at the O6?position of silyl?protected inosine, guanosine, and 2??deoxyguanosine is described. Ty

Abstract Table of Contents Materials Figures Literature Cited Abstract The conversion of 3?,5??disilylated 2??O?(methylthiomethyl)ribonucleosides to 2??O?(phthalimidooxymethyl)ribonucleosides is achieved in

Abstract Table of Contents Materials Figures Literature Cited Abstract High?content functional protein microarrays, such as ProtoArray Human Protein Array from Life Technologies, contain thousands of prot

Abstract Table of Contents Materials Figures Literature Cited Abstract In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein?protein interactions in situ

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the ChIP?exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion f

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for quantifying various cellular features (e.g., volume, total and subcellular fluorescence localization)