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Abstract Table of Contents Figures Literature Cited Abstract This unit describes critical components and considerations required to study protein?protein structural interactions inside a living cell by using NMR spectroscopy (STINT?NMR). S

Abstract Table of Contents Materials Figures Literature Cited Abstract Hepatitis C virus (HCV) affects an estimated 3% of the population and is a leading cause of chronic liver disease worldwide. Since HC

Abstract Table of Contents Materials Figures Literature Cited Abstract The kinetics of the ligand?receptor interaction is an important feature in lead optimization for new drug candidates. The protocol de

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptide?oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypepti

Abstract Table of Contents Materials Figures Literature Cited Abstract The detailed synthetic protocol for a 2?selenothymidine phosphoramidite and its use in preparing Se?derivatized oligonucleotides are

Abstract Table of Contents Materials Figures Literature Cited Abstract Metabolic oligosaccharide engineering (MOE) refers to a technique where non?natural monosaccharide analogs are introduced into living

Abstract Table of Contents Literature Cited Abstract Inhibitors of specific cellular pathways are useful for investigating the roles of proteins of unknown function, and for selectively inhibiting a protein in complex pathways to uncover its relationships to other proteins

Abstract Table of Contents Materials Figures Literature Cited Abstract Recent development of methods for genome?wide identification of transcription factor binding sites by chromatin immunoprecipitation (

Abstract Table of Contents Figures Literature Cited Abstract In addition to the orthosteric site, which recognizes endogenous ligands, most G protein?coupled receptors (GPCRs) possess topographically distinct allosteric sites that can be r

Abstract Table of Contents Materials Figures Literature Cited Abstract Cyclic nucleoside phosphonates connected through a P?O?C linkage to a promoiety represent a class of prodrugs designed to overcome th

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation of alkylthioalkylated and formamidoalkylated alcohols, an amidoalkylated alcohol, a hydroxylalkylat

Abstract Table of Contents Materials Figures Literature Cited Supplementary Material Abstract Supported membranes, formed as a single conti

Abstract Table of Contents Materials Figures Literature Cited Abstract Imaging of RNAs in single cells revealed their localized transcription and specific function. Such information cannot be obtained fro

Abstract Table of Contents Materials Figures Literature Cited Abstract Allosteric interactions involve the simultaneous binding of two ligands to the same receptor. An allosteric modulator causes a confor

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a simple procedure for preparing short DNA duplexes that contain a single N4C?alkyl?N4C interstrand cross?link. The

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes two methods for preparing oligonucleotides containing an O6?2??deoxyguanosine?alkyl?O6?2??deoxyguanosine interstran

Abstract Table of Contents Materials Figures Videos Literature Cited Abstract Protein microarrays offer a global perspective on the function of expressed gene products. Ho

Abstract Table of Contents Literature Cited Abstract Synthetic custom peptides offer a rational, scalable, and ever more affordable approach for exploring protein?protein interactions or even more complex phenomena, such as immune responses directed against specific epito .

Abstract Table of Contents Materials Figures Literature Cited Abstract Determination of the binding specificity of glycan?binding proteins (GBPs), such as lectins, antibodies, and receptors, has tradition

Abstract Table of Contents Materials Figures Literature Cited Abstract DamIP is a new method for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. c