实验仪器使用技术

Rotor equilibration without counterbalanceContributed by Dr.A.GratchevThis is relatively obvious method which I found not used by most of the researchers. Usually the equilibration of 234689 and 10 tu ...

Radioactive DecayIsotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable however radioisotopes are unstable and disintegrate ...

A. Gilson Pipetmen Autopipets (and similar models)We primarily use the Gilson autopipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The va ...

I. Safety Procedures A. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by law to supply the user with pertinent information ...

Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors the main disadvantage is ...

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide. Decontamination of solutions 0.5mg/mlThis method reduc ...

Log in radioactive material received and deduct amount of radioactive material used in each experiment in Radioisotope Log Book. Clear your bench top work area and make sure it is clean. Put on your l ...

RULES FOR THE USE OF RADIOACTIVITY ...

Silane and Poly-L-Lysine Coating of Microscope Slides1) Wash slides in Decon solution for 30 minutes. 2) Wash slides in running tap water for 30 minutes. 3) Wash slides in distilled water for 2 x 5 mi ...

This program helps you to calculate the concentration of nucleic acids according to their optical density. (DNA RNA oligonucleotides). It is better to use {OD260-OD320} instead of OD260. For a series ...

Plate assay for determination of DNA concentrationA fairly accurate rapid assay of DNA concentration can be obtained by UV visualization of samples spotted onto ethidium bromide-containing agarose pla ...

Characterization of dnaLEVEL I Materials DNA sampleSSC bufferUV spectrophotometer 3 and quartz cuvettes Procedure Dissolve a small quantity of your extracted DNA in 3.0 ml of 0.1X SSC. Turn on and bla ...

Quantitation of DNAFluorescent quantitation Wear gloves and goggles protecting from EtBr and UV light With an EDP (dilute mode) pick up 9 µl TE and 1 µl vortexed DNA sample. Spot this on a place of sc ...

Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic so ...

Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be ...

Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = micrograms mcL = microliters BSA = bovine serum albumin O.D. = optical density dI = deionized BackgroundThe Bradford protein a ...

Bradford � Protein DeterminationIntroduction A rapid and accurate method for the estimation of protein concentration. The technique is simpler faster than the Lowry method and is subject to less inter ...

Protein AssaysBelow is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range volume/amount of sample needed subjective comments on acc ...

Biorad Protein Assay: Bradford Standards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 µl 2 µg/ml 780 µl40 µl 4 µg/ml 760 µl60 µl 6 µg/ml 740 µl80 µl 8 µg/ml 720 µl100 µl 10 µg ...

BIURET PROTEIN ASSAYMATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard dilutions of BSA containing 1 2.5 5.0 7.5 and 10 mg/ml protein. Prepare s ...