实验试剂 1. Equilibrate sterile dH2O water or 10 mM Tris pH 9.0 at 65℃.2. Isopropyl alcohol (isopropanol; absolute)3. Absolute (96%-100%) ethanol4. RNase A stock solution at 20 mg/mL 5. Liquid nitrogen f ...
实验原理 The E.Z.N.A.® MicroElute Genomic DNA Kit uses the reversible binding properties of the HiBind® matrix a new silica-based material combined with the MicroElute column spin technology which allows ...
实验试剂 1. 80% Ethanol2. Isopropanol 3. Chloroform 实验设备 1. Magnetic Stand for 1.5 ml tube (OBI # MSD-02)2. Nuclease-free 1.5 and 2 ml centrifuge tubes 3. Microcentrifuge capable of 12000 x g and 2- ...
实验试剂 1. Absolute (96%-100%) ethanol 2. Molecular biology grade water 实验设备 1. Multiple-channel pipettor2. 96-well microplate 3. Magnetic Separation Devices 实验步骤 1. Determine the volume of ...
实验试剂 Regents supplied by user1. Sterile Deionized Water (or TE Buffer)2. 70% Ethanol 3. 96%-100% Isopropanol 实验设备 Equipments supplied by user1. Microcentrifuge capable of at least 18000 x g (a c ...
实验设备 Water bath at 37°CLaminar flow hoodPipet-aid pipettes and micropipettesMultichannel pipettes and repeater pipettePolystyrene round-bottom tubes (40mL) and petri dishes (92 x 17 mm) or similar co ...
实验试剂 1. 2.55 M sucrose (unbuffered) 1)Heat on a stirring block to dissolve then bring up to 1 L. 2)Determine concentration using a refractometer and adjust to 2.55 M sucrose (refractive in ...
实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples for direct use in reverse transcription PCR. The direct mRNA isolation is comp ...
实验试剂 柱材Oliga(dT)-纤维素上样液:20mM Tris-HCL(pH 7.6) 0.5M NaCL 1mM EDTA (pH 8.0) 0.1% 十二烷基肌氨酸钠(SLS)洗脱液:10mM Tris-HCL(pH 7.6) 1mM EDTA (pH 8.0) 0.05% SDS ...
实验原理 正常红细悬浮于等渗的血浆中,若置于高渗溶液内,则红细胞会因失水而皱缩;反之,置于低渗溶液内,则水进入红细胞,使红细胞膨胀。如环境渗透压继续下降,红细胞会因继续膨胀而破裂,释放血红蛋白,称之为溶血。红细胞膜对低渗溶液具有一定的抵抗力,这一特征称为红细胞的渗透脆性。红细胞膜对低渗溶液的抵抗力越大,红细胞在低渗溶液中越不容易发生溶血,即红细胞渗透脆性越小。将血液滴入不同的低渗溶液中,可检查红 ...
实验原理 很多植物受到机械损伤时在空气中会逐渐变成褐色,这是损伤时植物细胞破碎,原来彼此分开的多酚氧化酶和多酚类物质接触反应的结果。反应如下:+H2O多酚氧化酶+1/2O2多酚氧化酶(po1yphenoloxidase,PP0)是一种含铜酶,它能够催化酚类物质转变成醌。近几十年来,国内外对植物组织褐变的研究表明,组织的褐变主要是PP0作用于天然底物酚类物质所致。 实验试剂 1. 0.0 ...
实验原理 不少药物当以气雾法给予豚鼠时可以引起支气管痉挛、窒息,从而导致抽搐而跌倒。这种动物模型可用于观察药物的支气管平滑肌松弛作用。目前最常用的引喘药物是组胺和乙酰胆碱。 实验试剂 引喘药物: 组胺和乙酰胆碱实验药物: 氨茶碱或特布他林 实验设备 药物气雾引喘装置 实验材料 动物:豚鼠4只 实验步骤 1. 取豚鼠6只备用。2. 取豚鼠1只,放入玻璃罩内( ...
实验材料 NameCompanyCatalog NumberComments10 ml SyringeCole Parmer Instrument Co.07940-121-Way StopcoksCole Parmer Instrument Co.30600-0030 ml SyringeBD Medical3096504 -Way StopcocksCole Parmer Instr ...
实验试剂 1. pH值8.6、0.025mol/L巴比妥EDTA缓冲液(含0.01mol/L EDTA);2. 小牛胸腺DNA;3. pH值6.9、0.05mol/L PB;4. DNA酶溶液,每毫升pH值6.9、0.05mol/L PB中含MgCl2 0.003mol/L、1mg DNase;5. pH值5.3、0.3mol/L PB;6. pH值5.1、0.02mol/L PB(含0.01m ...
实验试剂 1.1X SDS gel-loading buffer50 mM Tris-Cl (pH 6.8)100 mM dithiothreitol2% SDS0.1% bromophenol blue10% glycerol1% SDS gel-loading buffer lacking dithiothreitol can be stored at room temperature. ...
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended th ...
实验试剂 1. Tris-Mg 缓冲液 10mM Tris-Cl 5mM MgCl2 pH 7.3,4℃保存 2. 2%(w/v)十二烷基肌氨酸钠 (SLS) 实验步骤 1. 于20ml 营养肉汤中过夜培养细菌,37℃,200rpm。 2. 10000g、20min、4℃离心,去上清。 3. 20ml预冷的Tris-Mg缓冲液重悬,同样条件离心,再重悬于预冷的 ...
实验试剂 1. RPMI-16402.Ⅰ、Ⅳ型胶原酶、DNA酶3. 0.001%的透明质酸酶和DNA酶 4. IL-2 实验设备 80目和200目不锈钢网 实验材料 肿瘤组织 实验步骤 1. 无菌切取肿瘤组织或肿瘤浸润的局部淋巴结,置于含抗生素的RPMI-1640培养液中;2. 将瘤体机械法剪碎,用RPMI-1640调整体积为10~20ml,同时加入0.05%的Ⅰ、 ...
实验原理 E.Z.N.A.TM FFPE DNA Kit uses the reversible binding properties of HiBind® matrix a new silica-based material combined with the speed of mini-column spin technology. A specifically formulated buf ...
实验材料 Contents and storage information. MaterialAmountConcentration StoragundefinedStabilityVybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain400 μL5 mM solution in dimethyl sulfoxide (D ...