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Synthesis of Building Blocks and Oligonucleotides with {G}O6‐Alkyl‐O6{G} Cross‐Links

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes two methods for preparing oligonucleotides containing an O6?2??deoxyguanosine?alkyl?O6?2??deoxyguanosine interstran

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Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies

Abstract Table of Contents Materials Figures Videos Literature Cited Abstract Protein microarrays offer a global perspective on the function of expressed gene products. Ho

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Storage and Handling Guidelines for Custom Peptides

Abstract Table of Contents Literature Cited Abstract Synthetic custom peptides offer a rational, scalable, and ever more affordable approach for exploring protein?protein interactions or even more complex phenomena, such as immune responses directed against specific epito .

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Preparation and Analysis of Glycan Microarrays

Abstract Table of Contents Materials Figures Literature Cited Abstract Determination of the binding specificity of glycan?binding proteins (GBPs), such as lectins, antibodies, and receptors, has tradition

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DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo

Abstract Table of Contents Materials Figures Literature Cited Abstract DamIP is a new method for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. c

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In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry

Abstract Table of Contents Materials Figures Literature Cited Abstract The ability to study proteins and protein interactions inside cells and tissues is important for elucidating how cells function in he

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Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applicati

Abstract Table of Contents Materials Figures Literature Cited Abstract Nature possesses an unlimited number and source of biologically relevant natural glycans, many of which are too complicated to synthe

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Analysis of Multidimensional G‐Quadruplex Melting Curves

Abstract Table of Contents Figures Literature Cited Abstract Multidimensional ?3D? melting curves for G?quadruplexes are obtained by recording whole spectra (absorbance, CD, fluorescence) as a function of temperature, rather than the commo

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Resolution of Quadruplex Polymorphism by Size‐Exclusion Chromatography

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for separation of quadruplex species formed from the same sequence via size?exclusion chromatography (SE

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Use of In Vivo Biotinylation for Chromatin Immunoprecipitation

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a system for expression of biotinylated proteins in mammalian cells in vivo, and its application to chromatin imm

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Simultaneous Single‐Molecule Mapping of Protein‐DNA Interactions and DNA Methylation by MAPit

Abstract Table of Contents Materials Figures Literature Cited Abstract Sites of protein binding to DNA are inferred from footprints or spans of protection against a probing reagent. In most protocols, sit

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Use of Chromophoric Ligands to Visually Screen Co‐Crystals of Putative Protein‐Nucleic Acid Complexes

Abstract Table of Contents Materials Figures Literature Cited Abstract Distinguishing between crystals of protein?nucleic acid complexes and those containing protein alone is a common problem in structura

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ICON Probes: Synthesis and DNA Methylation Typing

Abstract Table of Contents Materials Figures Literature Cited Abstract DNA methylation and demethylation significantly affect the deactivation and activation processes of gene expression, respectively. Th

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Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins

Abstract Table of Contents Materials Figures Literature Cited Abstract ?Regulator of G?protein Signaling? (RGS) proteins constitute a class of intracellular signaling regulators that accelerate GTP hydrol

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Oligodeoxynucleotide Containing S‐Functionalized 2′‐Deoxy‐6‐Thioguanosine: Facile Tools for Base‐Selective and Site‐Specific Internal Modification of

Abstract Table of Contents Materials Figures Literature Cited Abstract Chemically modified oligonucleotides play a significant role for genomic research. Modified nucleosides, such as with a fluorescent d

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Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides

Abstract Table of Contents Materials Figures Literature Cited Abstract Oligonucleotides are functionalized by conjugation with a variety of molecules, and aliphatic amino linkers have been frequently used

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High‐Throughput RT‐PCR for Small‐Molecule Screening Assays

Abstract Table of Contents Materials Figures Literature Cited Abstract Quantitative measurement of the levels of mRNA expression via real?time reverse transcription polymerase chain reaction (RT?PCR) has

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HPLC‐SEC Characterization of Membrane Protein‐Detergent Complexes

Abstract Table of Contents Figures Literature Cited Abstract Determination of the oligomeric state of integral membrane proteins in detergent solutions is a challenging task because the amount of detergent associated with the protein is ty

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Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR

Abstract Table of Contents Materials Figures Literature Cited Abstract In addition to X?ray, NMR, and FRET, electron paramagnetic resonance (EPR) can be applied to elucidate the structure of different mac

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Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

Abstract Table of Contents Materials Figures Literature Cited Abstract A simple method for the etherification at the O6?position of silyl?protected inosine, guanosine, and 2??deoxyguanosine is described. Ty

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