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- 详细信息
- 文献和实验
- 技术资料
- 适应物种:
Human,Mouse,Rat,
- 应用范围:
WB ELISA IHC-P IHC-F Flow-Cyt IF
- 抗体英文名:
Integrin Alpha V + Beta 3 (CD51+CD61)
- 规格:
50ul
| 英文名称 | Integrin Alpha V + Beta 3 (CD51+CD61) |
| 中文名称 | 整合素αVβ3抗体 |
| 别 名 | CD51 + CD61; CD51; CD61; GP3A; GPIIIa; integrin alpha v; integrin beta 3; intregrin alpha v beta 3; ITGAV; ITGB3; Platelet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; VNRA; Antigen identified by monoclonal antibody L230; CD51 antigen; CD61 antigen; DKFZp686A08142; Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin alpha v; Integrin beta 3 (platelet glycoprotein IIIa, antigen CD61); Integrin beta chain beta 3; intregrin alpha v beta 3; ITGAV; MSK8; Platelet glycoprotein IIIa; Platelet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; Vitronectin receptor subunit alpha; avb3. |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 3: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. Subcellular Location: Cell Membrane; single-pass type I membrane protein. SWISS: P05106, P06756 Gene ID: 3685 Database links: Entrez Gene: 3685 Human Entrez Gene: 3690 Human Omim: 173470 Human Omim: 193210 Human SwissProt: P05106 Human SwissProt: P06756 Human Unigene: 218040 Human Unigene: 436873 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 整合素αVβ3为二聚体的跨膜糖蛋白质,可以调节肿瘤细胞在多种细胞外基质蛋白中的粘附和迁移,在被激活的内皮细胞中有较高的表达,并在新生血管生成过程中发挥优势。 整合素αVβ3在细胞外的信号传入细胞内调节细胞生长、改变细胞形态、影响细胞运动, 并在肿瘤侵袭和转移的过程中起重要作用.整合素αVβ3是特异性表达在血管内皮细胞表面的粘附因子。 |
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文献和实验人整合素αVβ3(ITG αⅤβ3)酶联免疫分析(ELISA)
人整合素αVβ3(ITG αⅤβ3) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 整合素αVβ3(ITG αⅤβ3) 的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 人 整合素αV β 3 ( ITG αⅤβ 3 ) 水平。用纯化的抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 整合素αV β 3 ( ITG αⅤ
整合素家族迟现抗原组 ( β 1 组 ) β1(CD29)整合素亚单位可与CD49α~f(Mα1~α6)、CD51(αV)以及α7~α11分别组成VLAl~VLA6,αV61(VNR-191),α7α1、α8βl、α9β1和α11β1整合素分子。由于最早发现的~%A-1和VLA-2分子是在用同种异体抗原或外源凝集素活化淋巴细胞后数周才出现,因此习惯称为迟现抗原(verylateanngen,VLA)。CD29表达十分广泛,包括所有的白细胞。CD29在记忆T细胞上的表达水平高于
整合素家族血小板糖蛋白组 ( β 3 组 ) 和β 7 组 β3亚单位为CD61,分子质量为105kDa,表达于血小板、巨核细胞、单核细胞、Mφ和内皮细胞。CD6l可与CD4l和CD51分别组成血小板糖蛋白ⅡbⅢa(GPⅡbⅢa,αⅡbβ3)和VN受体(αVβ3)。其中的GPⅡbⅢa(CD61/C1341)在血小板受到凝血酶、腺苷二磷酸(ADP)和胶原等刺激活化后,即可与相应配体结合。而CD51/CD61是玻连蛋白受体(VNR),介导血小板黏附于固相化
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