- ￥428 - 1626
- 碧云天（beyotime）
- S0021
- 2025年09月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃避光保存，一年有效。4℃避光保存，半年有效。
- 规格：
500次/2500次
| 规格： | 500次 | 产品价格： | ￥428.0 |
|---|---|---|---|
| 规格： | 2500次 | 产品价格： | ￥1626.0 |
碧云天生产的一氧化氮检测试剂盒采用了经典的Griess Reagent，并对其测定的溶液体系进行了优化，使检测下限达到1μM，在1-100μM范围内有非常完美的线性关系。
检测速度极快，完成一条标准曲线或5-10个样品的测定只需3分钟。
样品范围广，可以检测细胞或组织及其培养液中的一氧化氮的含量，酚红和10%血清均对测定无明显干扰，也可以检测血清、血浆和尿液中一氧化氮的含量。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| S0021M-1 | 1M NaNO2 | 1ml |
| S0021M-2 | Griess Reagent I | 125ml |
| S0021M-3 | Griess Reagent II | 125ml |
| — | 说明书 | 1份 |
保存条件：
-20℃避光保存，一年有效。4℃避光保存，半年有效。
注意事项：
本产品对人体有害，操作时请小心，并注意有效防护以避免直接接触人体或吸入体内。
如保存不当导致溶液变色或沉淀，则说明该溶液已经失效，请购买新的试剂盒。
不建议使用RIPA裂解液对细胞或者组织进行裂解，使用RIPA裂解液可能在后续反应中产生沉淀，影响测试。推荐使用碧云天的细胞与组织裂解液(一氧化氮检测用)(S3090)或Western及IP细胞裂解液(P0013)。
对于血清样品中NO含量的测定，粗略地计算，可以直接用水稀释标准品，从而计算出血清样品中NO的浓度。比较精确地计算，如果测定的正常血清是常见血清可以从文献上查到其中NO的浓度，然后用该已知NO浓度的血清稀释标准品，这样就可以得到比较精确的NO浓度。或者使用已知浓度的人或其它动物的血清稀释标准品也同样可以达到目的。或者参照类似文献进行血清中NO浓度的测定。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
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HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3
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Dietary salt regulates epithelial sodium channels in rat endothelial cells: adaptation of vasculature to salt.
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J Microbiol Immunol. 2005; 3(4):260-5.(IF 3.493)
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Estrogen provides neuroprotection against activated microglia-induced dopaminergic neuronal injury through both estrogen receptor-alpha and estrogen receptor-beta in microglia.
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The antioxidative effects of astragalus saponin I protect against development of early diabetic nephropathy.
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Non-cell Corynebacterium parvum generated by nanotechnology: a promising immunomodulator with less side effects.
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The effect of exogenous nitric oxide on alleviating herbicide damage in Chlorella vulgaris.
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dl-3-n-Butylphthalide prevents oxidative damage and reduces mitochondrial dysfunction in an MPP(+)-induced cellular model of Parkinson's disease.
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Reducing nanotube cytotoxicity using a nano-combinatorial library approach.
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N-butanol Extract from Melilotus Suaveolens Ledeb Affects Pro- and Anti-Inflammatory Cytokines and Mediators.
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Protective Effect of Anthocyanins Extract from Blueberry on TNBS-induced IBD Model of Mice.
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Effects of daintain/AIF-1 on β cell dysfunction in INS-1 cells.
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ADAMTS-5 and intervertebral disc degeneration: the results of tissue immunohistochemistry and in vitro cell culture.
J Orthop Res. 2011 May;29(5):718-25.(IF 2.728)
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Inflammation. 2013 Aug;36(4):954-61.(IF 3.212)
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Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce apoptosis in porcine alveolar macrophage via increasing nitric oxide production, oxidative stress,and caspase-3 activation.
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3β-Angeloyloxy-8β,10β-dihydroxyeremophila-7(11)-en-,8α-lactone Inhibits Lipopolysaccharide-Induced Nitric Oxide Production in RAW264.7 Cells.
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Scand J Immunol. 2015 Oct;82(4):370-9.(IF 2.717)
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NITRIC OXIDE-BIOL CH. 2016 Jan 30;52:56-65.(IF 3.311)
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AAPS PharmSciTech. 2016 May 25. [Epub ahead of print](IF 2.401)
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