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- QIAGEN
- 47016
- 2026年01月08日
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| 品名 | 货号 | 品牌 | 规格 |
| Qiagen 47014 强力土壤基因组DNA提取试剂盒（50） | 47014 | Qiagen | 50T |
| Qiagen 47016强力土壤基因组DNA提取试剂盒（250） | 47016 | Qiagen | 250T |
Introduction
The DNeasy PowerSoil Pro Kit comprises a novel and proprietary method for isolating microbial genomic DNA from environmental samples. The kit uses QIAGEN’s second- generation Inhibitor Removal Technology® (IRT) and is intended for use with environmental samples containing high humic acid content, including difficult soil types such as compost, sediment, and manure. Other more common soil and stool types have also been used successfully with this kit. Improved IRT combined with more efficient bead beating and lysis chemistry yields high-quality DNA that can be used immediately in downstream applications, including PCR, qPCR, and next-generation sequencing (16S and whole genome).
| DNeasy PowerSoil Pro Kit Catalog no. Number of preps |
(50) 47014 50 |
(250) 47016 250 |
| PowerBead Pro Tubes | 50 | 250 |
| MB Spin Columns | 50 | 250 |
| Solution CD1 | 40 ml | 200 ml |
| Solution CD2 | 15 ml | 60 ml |
| Solution CD3 | 35 ml | 175 ml |
| Solution EA | 36 ml | 175 ml |
| Solution C5 | 30 ml | 175 ml |
| Solution C6 | 9 ml | 66 ml |
| Microcentrifuge Tubes (2 ml) | 100 | 500 |
| Elution Tubes (1.5 ml) | 50 | 250 |
| Collection Tubes (2 ml) | 100 | 500 |
| Quick-Start Protocol | 1 | 1 |
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文献和实验A260值代表更多DNA 错。高A260值并不总是意味着高基因组DNA得率。除了基因组DNA本身，降解的DNA、RNA同样是高UV吸光率的。降解的RNA的高吸光率推高了A260的读数。样品裂解完成后采用什么DNA纯化方法决定了最终获得什么样的DNA。很多纯化方法并不能从大分子DNA中分离掉小片段DNA、RNA。而PowerSoil DNA Isolation Kit可以做到。 跑胶5-10 μl可以让你了解你的DNA样品有什么。从电泳结果上能清楚看到样品主要是大分子DNA，还是破碎不堪的小片段核酸
the anti-Flag-M2 beads. Response: This has been changed accordingly and the saved input is now in step 14 of Part-II. 5.For preparation of genomic DNA by Qiagen DNeasy kit, guanidine hydrochloride would be used at the lysis step. Can DNA (or RNA)-siRNA
Plant DNA methylation analysis by bisulfite genomic sequencing
and Wassenegger (2000). Back to top Procedure Preparation of Plant Genomic DNA Prepare plant genomic DNA using a Qiagen DNeasy Plant Maxi or Mini kit (see comment 1). Prior to the bisulfite-treatment, the plant genomic DNA is cleaved
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