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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Nucleocapsid (E378Q) Protein (His Tag)
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素Please contact us for more information.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid (YP_009724397.2, with mutation E378Q) (Ser2-Ala419) was expressed with a polyhistidine tag at the N-terminus.
表达宿主E. coli
种属SARS-CoV-2
预测 N 端Met
分子量The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid consists of 425 amino acids and predicts a molecular mass of 46.5 kDa.
缓冲液Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
ligonucleo t ide m icroch ip )。 其利用杂交测序(sequencing by hyb ridiza2tion, SBH) 原理, 在载体表面建立可寻址的高密度DNA 分子微阵列, 通过与标记过的样品核酸序列互补匹配, 进行测序或生物基因信息的大规模平行检测。2 研究背景生物芯片的概念来自计算机芯片, 是20 世纪80 年代初提出来的, 最初主要指分子电子器件。 目前意义上的生物芯片是90 年代初提出来的, 该概念的提出与人类基因组计划(hum an genom e p ro ject
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