- ￥618
- 翌圣生物（Yeasen）
- 10620ES10
- 上海翌圣
- 2025年10月30日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
UCF·ME Pyrophosphatase,Inorganic GMP-grade
- 保质期：
有效期1年
- 供应商：
翌圣生物科技(上海)股份有限公司
- 保存条件：
干冰运输。-20℃保存
- 规格：
10U
Product Information
| Product Name |
Catalog No. |
Size |
Price (￥) |
| Pyrophosphatase, Inorganic GMP-grade (1 U/μL) |
10620ES10 |
10 U |
645 |
| 10620ES60 |
100 U |
4855 |
|
| 10620ES80 |
1,000 U |
Inquiry |
|
| 10620ES99 |
40 KU |
Inquiry |
Product Description
This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.
This product is produced in accordance with GMP process requirements, and the product is provided in liquid form
Product Properties
| Source |
Recombinant E.coli with Pyrophosphatase gene from yeast |
| Optimum Temperature |
25℃ |
| Storage Buffer |
50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin |
| Unit Definition |
One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min） |
Contents
| Catalog No. |
Name |
Catalog No./Specification |
|||
| 10619ES10 (10 U) |
10620ES60 (100 U) |
10620ES80 (1,000 U) |
10620ES99 (40 KU) |
||
| 10620 |
Pyrophosphatase, Inorganic GMP-grade (1 U/μL) |
10 μL |
100 μL |
1 mL |
40 mL |
Shipping and Storage
The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Notes
1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.
3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this
HB220514
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文献和实验磷酸酶的一种。是催化焦磷酸水解为正磷酸的酶之总称。EC 3． 6． 1． 1．仿照磷酸单酯酶，根据最适 pH可分为三种情况：（ 1）在最适 pH为 7.2-7.8，可通过 Mg2 而被活化，含于动物组织和酵母中；（ 2）最适 pH5.0-5.5，含于肝脏、肾脏、霉菌和植物的种子中不为金属盐所活化，可因氟化物而受阻抑；（ 3）最适 pH3.2-4.0，含于肝脏、曲霉和酵母中。
核苷酸焦磷酸酶nucleotide pyrophosphatase
EC3． 6． 1． 9。为催化切断 NAD ， NADP ， FAD， ATP， ADP，硫胺素二磷酸等的核苷酸焦磷酸键反应的酶。对无机焦磷酸和磷酸单酯无作用。广泛存在于生物界中。
无机物是无机化合物的简称，通常指不含碳元素的化合物。少数含碳的化合物，如一氧化碳、二氧化碳、碳酸盐、氰化物等也属于无机物。无机物大致可分为氧化物、酸、碱、盐等。
