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- Tanda
- TD-AB0097
- 2025年12月17日
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- 技术资料
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 抗体英文名：
mitochondrial calcium uptake 1
- 抗体名：
mitochondrial calcium uptake 1
- 规格：
25 μl/100 μl/200 μl
MICU1 mouse monoclonal antibody
| Product Name | MICU1 mouse monoclonal antibody |
| Cat.# | TD-AB0097 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti- MICU1 mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of human MICU1 |
| Reactivity | Human |
| Host species | Mouse |
| Ig class | |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | MICU1 |
| Full name | mitochondrial calcium uptake 1 |
| Synonyms | CALC; EFHA3; MPXPS; CBARA1; ara CALC |
| Target Background | |
| This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. | |
| Western blotting | |
| Predicted band size | 54 kDa |
| Positive control | A549, Hela, K562 and 231 cell lysates |
| Recommended dilution | 1000-5000 |
| Gel | 12%SDS-PAGE |
| Lysate | 40 μg |
| Lane 1-4 | A549, Hela, K562 and 231 cell lysates |
| Primary antibody | D191077(MICU1 Antibody) at dilution 1/1200 |
| Secondary antibody | Goat anti mouse IgG at 1/5000 dilution |
| Exposure time | 10 seconds |
| Product Image | |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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