- 询价
- Tanda
- TD-AB0011
- 中国
- 2025年12月15日
- WB
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Flag tag mouse monoclonal antibody
- 抗体英文名：
Flag tag mouse monoclonal antibody
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
脊椎动物
- 保质期：
1年
- 抗原来源：
Mouse
- 级别：
科研级
- 库存：
800
- 供应商：
武汉天德
- 克隆性：
单克隆
- 保存条件：
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
- 形态：
液体
- 亚型：
IgG2a
Flag mouse monoclonal antibody
Cat.# TD-AB0011
Package 25 μl/100 μl/200 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-flag mouse monoclonal antibody
Applications WB
Immunogen Synthetic peptide conjugated with KLH
Reactivity Fusion proteins containing the Flag Tag
Host species Mouse
Ig class IgG2a
Purity Protein A/G Affinity Purification
Target information
Symbol Flag tag
Full name Flag tag
Target Background
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins
Western blotting
Predicted band size 50 kDa
Positive control Fusion proteins containing the Flag Tag
Recommended dilution 1000-5000
Gel: 8%SDS-PAGE
Lysate: 0.08/0.16/0.32 μg
Lane 1-3: Fusion proteins containing the Flag Tag
Primary antibody: Flag tag Antibody at dilution 1/1200
Secondary antibody: Goat anti mouse IgG at 1/5000 dilution
Exposure time: 3 seconds
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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