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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Flag-Tag Mouse Monoclonal Antibody
- 抗体英文名:
Flag-Tag Mouse Monoclonal Antibody
- 靶点:
详见说明书
- 浓度:
1.0mg/ml
- 应用范围:
WB ICC/IF IP
- 宿主:
Mouse
- 供应商:
上海钰博生物科技有限公司
- 库存:
大量
- 目录编号:
0008
- 抗原来源:
Mouse
- 保质期:
1年
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20°C
- 形态:
液体或冻干粉
- 亚型:
详见说明书
- 免疫原:
详见说明书
- 规格:
50μg/50μl, 100μg/100μl
-
- Catalog No. : AB0008
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : N/A
- UniProt : N/A
-
- Isotype : Mouse IgG1
- Clonality : Monoclonal
-
- Price : $165/100μg
- Availability : Ship next day
-
- Rank:
- Download datasheet [PDF] :
- Rank:
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
N/A
Application:
WB ICC/IF IP
Reactivity:
All
Dilution:
WB 1:3000~1:10000 ICC/IF 1:100~1:200 IP 1:100
![]() |
Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. |
Purification:
Affinity-chromatography
Specificity:
Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins.
Immunogen:
A synthesized peptide
Description:
Flag-Tag antibody is part of the Tag series of antibodies, the best quality in the research.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -24°C long term. Avoid freeze / thaw cycle.
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
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