IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
浓度:
1mg/ml
靶点:
Rab35
抗体英文名:
Anti-Rab35-GTP Monoclonal Antibody
抗体名:
Rab35-GTP
规格:
30 μL
Rab35-GTP
Cat. #: TD-26922
Size: 30 µL
Gene Symbol: RAB35
Description: Anti-Rab35-GTP Monoclonal Antibody
Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
Immunogen: Recombinant full length protein of active Rab35
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:
Rab35 Activation Assay.Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. TD-26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. ).