- ￥4800
- Tanda
- 美国
- TD-26474
- 2025年10月29日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26474
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
G13R
- 抗体英文名：
Anti-Ras (G13R) mouse monoclonal antibody
- 抗体名：
Ras (G13R)
- 规格：
100 μL
RAS(G13R)
| Cat. #: TD-26474 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-RAS(G13R) Mouse Monoclonal Antibody |
| Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G13R mutant, but not wild type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant HRAS(G13R) and wild type proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(G13R) monoclonal antibody (Cat.#TD-26474).
Immunofluorescence:  
Immunofluorescence of cells expressing Ras proteins with Anti-RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13V) plasmid, pCDNA3-GFP-Ras (G13A) plasmid, or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with Anti-RAS(G13R) monoclonal antibody (Cat. #TD-26474).
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文献和实验Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
active form of Ras -related G proteins involved immunoprecipitation of 32 P-labeled protein followed by separation of the labeled GTP/GDP bound to GTPase. A new method based on the large affinity difference of the GTP- and GDP-bound form of Ras proteins
Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors
A complex intracellular signaling network mediates the multiple biological activities of G-protein-coupled receptors (GPCRs). Among them, monomeric GTPases and a family of closely related proline-targeted serine–threonine kinases
Figure G.1 Electromagnetic Radiation Spectrum Figure G.2. Schematic light transmission Figure G.3. Use of the Spec 20 A spectrophotometer or colorimeter makes use of the transmission of light
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