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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26444
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IF, IHC
- 浓度:
1mg/ml
- 靶点:
M2011R
- 抗体英文名:
Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody
- 抗体名:
NOTCH1(M2011R)
- 规格:
100 μL
NOTCH1(M2011R)
| Cat. #: TD-26444 |
| Size: 100 µL |
| Gene Symbol: NOTCH1, AOS5, AOVD1,TAN1, hN1 |
| Description: Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody |
| Background: NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2. |
| Immunogen: A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes M2011R mutant, but not wild type NOTCH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: ![]() Western blot analysis of recombinant NOTCH1(M2011R) and wild type proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild type protein (lane2) were blotted with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #TD-26444). |
Immunofluorescence: ![]() Immunofluorescence of cells expressing NOTCH1 proteins with Anti-NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP-NOTCH1 (WT) plasmid (left column) or pCDNA3-GFP-NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #TD-26444). |
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文献和实验polo6892 请问哪个实验室的同学或是老师有Notch1质粒,有点朋友请和我联系下好吗?有事情咨询! 多谢!我的EMAIL :zhouwei5352877@163.com,QQ 286358161,我是武汉大学人民医院实验室的研究生! 谢谢! liudaifu1997 我也感兴趣,等 qinvicky 我们实验室做基因转染的,一直用N2质粒,不过我懂得不是很多~不知能否帮
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