- ￥4800
- Tanda
- 美国
- TD-26216
- 2025年10月30日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of PIK3CA which includes the mutation of H1047Y, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26216
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
0.5 mg/ml
- 靶点：
H1047Y
- 抗体英文名：
Anti PIK3CA(H1047Y) Mouse Monoclonal Antibody
- 抗体名：
PIK3CA(H1047Y)
- 规格：
100 μL
PIK3CA(H1047Y)
| Cat. #: TD-26216 |
| Size: 100 µL |
| Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
| Description: Anti-PIK3CA(H1047Y) Mouse Monoclonal Antibody |
| Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of H1047Y, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 0.5 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes H1047Y mutant, but not wild type PIK3CA of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
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Immunofluorescence:
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文献和实验过程相对简单，检测灵敏度较高；Western blotting操作过程繁琐，检测灵敏度较低。3、荧光素酶报告基因检测只需要成功将含有目标DNA序列插入到报告载体中，即可进行后续实验，Western blotting则需要购买商品化的一抗，一般价高量少，且抗体的杂交条件需要摸索；若自制的一抗，抗体效价需要验证，实验周期较长。六、参考文献Pan, Y., R. Geng, N. Zhou, G. F. Zheng, H. Zhao, J. Wang, C. M. Zhao, X. B. Qiu, Y. Q
Protocol for competitive RT-PCR
) in which the amount of standard mRNA equal to the amount of transcript RNA can be read at the intersection of the line with the y-value 1. Ideally, the 4 measured ratios should yield a straight line. However, because of variances due to pipetting, dilution etc, the ratios
xuchenmail 最近看了一篇文献，关于PIK3CA 基因突变的问题，文献说热突变位点在exon 9 (E542K, E545K) and exon 20 (H1047R)，但是我根据pubmed上的数据，H1047R已经在21号外显子了，这是怎么回事啊？是文献错了吗？还是有其他的原因？ zjubell 1.这篇文献是什么时候发表的？对序列更新也是很正常的事。另外，这篇文献IF是多少？ 2.是否存在可变
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