Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of H1047Y, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes H1047Y mutant, but not wild type PIK3CA of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Western blot:
Western blot analysis of recombinant PIK3CA(H1047Y) and wild type proteins. Purified His-tagged PIK3CA(H1047Y) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(H1047Y) monoclonal antibody (Cat. #TD-26216).
Immunofluorescence:
Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(H1047Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(H1047Y) plasmid (right column), then fixed and stained with Anti-PIK3CA(H1047Y) monoclonal antibody (Cat. #TD-26216).