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MEK1 (P264S)

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  • ¥4800
  • Tanda
  • 美国
  • TD-26266
  • 2025年10月26日
  • ELISA, WB, IHC
  • Mouse
  • 脊椎动物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      A synthetic peptide from the internal region of MEK1 which includes the mutation of P264S, human origin.

    • 亚型

      lgG

    • 形态

      液体

    • 保存条件

      -20℃

    • 克隆性

      单克隆

    • 标记物

      FITC

    • 适应物种

      脊椎动物

    • 保质期

      24个月

    • 抗原来源

      蛋白

    • 目录编号

      TD-26266

    • 级别

      科研级

    • 库存

      999

    • 供应商

      武汉天德

    • 宿主

      Mouse

    • 应用范围

      ELISA, WB, IHC

    • 浓度

      0.3 mg/ml

    • 靶点

      P264S

    • 抗体英文名

      Anti MEK1(P264S) Mouse Monoclonal Antibody

    • 抗体名

      MEK1(P264S)

    • 规格

      100 μL

    MEK1(P264S)

    Cat. #:  TD-26266
    Gene Symbol:  MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
    Description:   Anti-MEK1(P264S) Mouse Monoclonal Antibody
    Background:   MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.
    Immunogen:    A synthetic peptide from the internal region of MEK1 which includes the mutation of P264S, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
    Concentration:   0.3 mg/ml
    Host Species:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Preservative: No
    Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes P264S mutant, but not wild type MEK1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
    Western blot: 
     产品细节图片1
    Western blot analysis of recombinant MEK1(P264S) and wild type proteins. Purified His-tagged MEK1(P264S) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-MEK1(P264S) monoclonal antibody (Cat. #TD-26266). 
    Immunofluorescence: 
     产品细节图片2
    Immunofluorescence of cells expressing MEK1 proteins with Anti-MEK1(P264S) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1(P264S) plasmid (right column), then fixed and stained with Anti-MEK1(P264S) monoclonal antibody (Cat. #TD-26266).

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    图标文献和实验
    相关实验
    • COMBINED ALCIAN BLUE - P.A.S

        SECTIONS 4micron paraffin wax sections   SOLUTIONS 1. Alcian Blue pH 2.5   Alcian Blue 1g 3% Acetic Acid 100ml 2. 1% Periodic Acid 3. Schiffs Reagent   METHOD   Take sections

    • COMBINED ALCIAN BLUE - P.A.S

        COMBINED ALCIAN BLUE - P.A.S   SECTIONS 4micron paraffin wax sections SOLUTIONS 1. Alcian Blue pH 2.5   Alcian Blue 1g 3% Acetic Acid 100ml

    • 犬蛋白S(P-S)酶联免疫分析

      犬 蛋白S(P-S) 酶联免疫 分析 试剂 盒使用说明书 本试剂仅供研究使用          目的:本试剂盒用于测定犬血清,血浆及相关液体样本中蛋白S(P-S)的含量。 实验原理 :     本试剂盒应用双抗体夹心法测定 标本 中 犬蛋白S(P-S) 水平。用纯化的 犬蛋白S(P-S) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 蛋白S(P-S) ,再与 HRP 标记的蛋白 S(P-S) 抗体结合,形成抗体 - 抗原 - 酶标

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