- ￥4800
- Tanda
- 美国
- TD-26109
- 2025年10月29日
- ELISA, WB, IHC, IP
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of Braf (N581S), human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26109
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC, IP
- 浓度：
0.5 mg/ml
- 靶点：
N581S
- 抗体英文名：
Anti BRaf(N581S) Mouse Monoclonal Antibody
- 抗体名：
B-Raf(N581S)
- 规格：
100 μL
BRAF(N581S)
| Cat. #: TD26109 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti-BRaf(N581S) Mouse Monoclonal Antibody |
| Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: A synthetic peptide from the internal region of Braf (N581S), human origin. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:100-200 |
| Concentration: 0.5 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes B-Raf N581S, but not wild type B-Raf proteins from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:
Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(N581S) monoclonal antibody (Cat. # TD-26109).
Immunofluorescence: 
Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(N581S) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(N581S) plasmid (right column), then fixed and stained with Anti-BRaf(N581S) monoclonal antibody (Cat. #TD-26109).
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