Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen: A synthetic peptide from the internal region of β-catenin which includes the mutation of G48D, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes β-catenin (G48D), but not wild type β-catenin protein from vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Western blot:
Western blot analysis of recombinant β-catenin (G48D) and wild type proteins. Purified His-tagged β-catenin (G48D) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti- β-catenin (G48D) mouse monoclonal antibody (Cat. #TD-26166). Immunofluorescence:
Immunofluorescence of cells expressing β-catenin proteins with anti- β-catenin (G48D) antibody. HEK293T cells were transfected with pCDNA3-GFP-β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin (G48D) plasmid (right column), then fixed and stained with anti- β-catenin (G48D) monoclonal antibody (Cat. #TD-26166).