SNARe Plasmid DNA Purification

Extraction and Purification of Plasmid DNA

Plasmids may be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. Many of these are rapid, small-scale “minipreps” that may be used effectively

Purification of Supercoiled Plasmid DNA

The topology of DNA affects a number of major biological processes (1 -4 ). For example, processive enzymes, such as the RNA and DNA polymerases, have the capability of generating both positive and negative DNA supercoils during the process

Large-Scale, Nonchromatographic Purification of Plasmid DNA

A large-scale approach to the purification of plasmid DNA has been developed that overcomes many of the limitations of current chromatography-based processes. The process consists of a scaleable lysis using recombinant lysozyme and a rapid