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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
ROOM TEMPERATURE
- 供应商：
西宝生物
- 库存：
100/box
- 英文名：
10 WELL MULTIWELL SLIDES, 5 mm
- 规格：
LABWARE
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文献和实验Multiwell Transformation Protocol
Multiwell Transformation Protocol Labor-saving implements Multichannel pipettors--1 to 25 µl and 20 to 200 µl volume (Brinkmann 12-channel). Sterile 96-well assay or tissue culture plate with lid
RUVKUN Antibody Staining Protocol revised 10/29/90
): 7. Incubate in BO3 buffer + 10 mM DTT, 15 minutes, 37 C with agitation. Wash once in 1X BO3 buffer. OXIDIZE -SH GROUPS TO -SO3: 8. Incubate in BO3 buffer + 1% H2O2, 1 hour, RT. Agitate gently but keep tubes upright because cap can pop open
RNA FISH on cultured cells in interphase
on the cell type. RNA is vulnerable to degradation at this step, therefore Vanadyl Ribonucleoside Complex (10mM final concentration) can be included in the extraction buffer; Incubate slides for 30 seconds in CSK buffer at room temperature; Fix
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