β-ENDORPHIN, Fragment 1-27

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

SUPPLEMENTARY DATA Supplementary Material REFERENCES RESULTS Designing the tools for assisted large fragment insertion The pSC101BADγβαA[hygro] vector

Enzyme Immunoassay of Urinary -Core Fragment of Human Chorionic Gonadotropin as a Tumor Marker for Ovarian Cancer

represents hCGβ-core fragment (β-CF) ( 2 ). Urinary β-CF consists of residues 6-40 disulfide bridged to residues 55-92 of the β-subunit of hCG ( 3 , 4 ), suggesting that it may be a degradative product of hCGβ. Such partial identity of the amino acid sequence

A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Inte

“cut and paste” techniques, combined with PCR. Here we describe a simple and general procedure to specifically insert a DNA fragment into any site within a given DNA sequence. We demonstrate this insertional mutagenesis by describing the insertion