Human Total Tau Assay Kit

Human DC Enrichment Kit

in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2.        Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1)        Dilute 10 – 18 ml buffy coat with Buffer 2 to a total

ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit

complex from step 10 above. Vortex briefly to mix and incubate at room temperature for 15 minutes with rotation. Spin down agarose, and carefully transfer the supernatant fraction (eluate) to another tube and repeat elution. Combine eluates (total

Cell-Based Assays for Regulators of Tau Biology

portray this cellular landscape. While we cannot ask many mechanistic questions using the human brain as our test subject, cell culture techniques that have emerged at least provide us with the ability to pose fundamental biochemical questions