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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
Cell-Based Assays for Regulators of Tau Biology
portray this cellular landscape. While we cannot ask many mechanistic questions using the human brain as our test subject, cell culture techniques that have emerged at least provide us with the ability to pose fundamental biochemical questions
Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation
to these activities in human liver microsomes (9 , 15 –18 ). Recently, lauric acid 11 -hydroxylation was identified as an alternative probe for human hepatic CY2El (19 ). However, an advantage of using p-nitrophenol hydroxylation to measure CYP2E1 activity
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