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Syringes and Hand Vacuum PumpsUse polypropylene syringes to dispense liquids into small containers and pressure-filter small volumes through filter holders or filter units with female Luer slip inlets. Syringes have male Luer slip outlet. An accessory 2-way vacuum valve, nylon male-to-male Luer adapter and rubber tubing with male Luer inlet, convert a standard syringe into a hand vacuum pump. |
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详细描述见链接:http://www.millipore.com/catalogue/item/XX6200036
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文献和实验E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
. It is recommended to homogenize the sample with rotor-stator homogenizers since it normally produce better yield. 2. Spin at 5,000 x g for 10 min at room temperature. Transfer the supernatant into a new tube and add an equal volume (2ml or 4ml) 70% Ethanol
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
QIAgene Expression Kit——再也不为重组蛋白的表达效率发愁 据Protein Database的最新数据表明,目前已知的人类蛋白有35,000种(包括splice variants),大约33%的蛋白正处于研究中,但是只有8%的蛋白能够成功表达。统计数据同时显示,目前克隆,表达,纯化的成功率只维持在54%,74%,59%。所以说,在蛋白表达的漫漫征途中,拿不到想要的蛋白是一件非常普遍的事情。举一个例子来说对于目标蛋白不表达或表达量低这个常见问题,需要排除或检查的因素就太多
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