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- 2025年07月05日
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Fittings, Adapters, Valves, Gauges and Tubing |
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文献和实验. Keep the samples for at least 2 hours at -80°C. 78. Centrifuge the samples at 13,000 rpm in a microcentrifuge for 30 minutes. Carefully discard the supernatant. 79. Rinse each pellet with 1 ml of 70% (v/v) ethanol. 80
In Situ Hybridization Protocols
minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age of the embryo. Wash once in DEPC-PBS at room
Anderson Lab In Situ Hybridization Protocols
in 4% paraformaldehyde in DEPC-PBS at room temperature for 20 minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age
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