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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Protein Purification
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
10
95.25 mm x 50.8 mm x 91.44 mm
(3.75 in. x 2 n. x 3.6 in.)
Product Family Information
Lynx® ST ConnectorsEnables integration of steamable hard piped process equipment with disposable sterile fluid paths |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/STC21THN10
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文献和实验; M. pirum) PCR reagents : Taq polymerase ( 5 U / ul ) dNTP( dGTP, dCTP, dTTP, dATP, 1.25 mM each ) 10x PCR buffer (with 1.5mM MgCl2) 25mM MgCl2 sterile ddH2O Machine / Equipment: PCR thermal cycler agarose电泳设备 DNA电泳胶体观察设备 无菌PCR反应管 无菌1.5 ml
Plant DNA methylation analysis by bisulfite genomic sequencing
(12000 X g); Discard supernatant, vacuum dry for 10 minutes at room temperature; Dissolve cut DNA in 10µl bidist water (0.25 or 2.5 µg/µl, respectively), store at -20°C; Bisulfite treatment The bisulfite solution
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
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