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文献和实验Cloning by Limiting Dilution of Hybridoma
according to the calculated dilutions. Serially dilute each clone to 4, 2, and 1 cell/ml. The final dilution tube should contain 50 ml of cloning medium at 1 cell/ml. Pour each of the dilutions into a sterile reagent reservoir. Plate 250 ml/well into 96-well plates
Construction and Characterization of Adenovirus Vectors
from Step 13) with 2 µL of PacI in a total volume of 50 µL overnight at 37°C. 16. Mix 40 µL of PacI-digested DNA with 360 µL of MEM and 16 µL of transfection reagent in a 5-mL tube. Vortex vigorously for 10 sec. 17. Allow
with the highest standards of humane care. 3) For isolation of endothelial progenitor cells, collect 50 ml of peripheral blood via standard phlebotomy technique from a consented volunteer donor into blood collection bags filled with the anticoagulant
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