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- S2EM004M99
- 2025年07月15日
- ELISPOT Assays
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- 应用范围：
ELISPOT Assays
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大量
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- 规格：
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MultiScreenHTS Filter Plates for ElispotThe ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases. |
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详细描述见链接：http://www.millipore.com/catalogue/item/S2EM004M99
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文献和实验into eppyincubate 45' 4℃ à Vortex several times duringspin 10 secs 4℃divide sample: ~400 l for IP/ ~ 100 l for WesternIP -- 1° Ab => 4℃ 1 hour rockingif 1° is not rab.à 2° Ab => 4℃ 1 hour rocking (RaM 2l)-- PrA/Seph 4℃ 1 hour rocking 40l-- Spin down 4℃ 1'-- Wash 3X
Transformation of Electrocompetent E. coli with Blue/White selection
to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed
Pollen Development in Anthers of Arabidopsis
Overview Using this procedure, it is possible to follow the development of pollen mother cells through to mature pollen. Sterile males
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