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- MSIPS4W10
- 2025年07月09日
- ELISPOT Assays
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ELISPOT Assays
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MultiScreen Filter plates with Durapore® MembraneMultiScreen plates with Durapore membrane are available in multiple pore sizes and with either hydrophilic or hydrophobic membranes. Plates are commonly used for general sample prep, purification of beads for Luminex assays, neonatal screening and Elispot assays. |
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MultiScreenHTS Filter Plates for ElispotThe ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases. |
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MultiScreen Plate OverviewDeveloped to meet the needs of our customers, MultiScreen plates are proven as optimized, reliable tools for the advancement of drug discovery and life science research. We are committed to providing a premium quality product coupled with a high level of technical support. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/MSIPS4W10
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文献和实验关于ELISPOT试剂盒: 用Mabteck公司的ALP标记抗体吧，配合Millipore公司的Multiscreen-IP 96孔板，所有的试剂都可以减半，照样可以出非常好的结果，4*96孔的试剂盒我一直做8*96孔，但BCIP/NBT显色液没试过减半，因为它很便宜。记得当年第一次做实验就出了很好的结果，经过不断摸索，做出的蓝紫色斑点确实非常漂亮，几乎没有非特异斑点，嗯，ELISPOT是我最拿手的实验之一。 关于PBMC: 拿到血液标本后，首先我要做的是尽快分离PBMC（一般2h
protocol。综合了 nature protocol（Guidelines for the automated evaluation of Elispot assays），CTL，mabtech 和其他若干 paper 的经验，写下了这篇标准 protocol，一步步走向成功。以检测 IFN－γ 为例，本人已经做了 20 多次，屡试不爽，先 show 几张图吧：ELISPOT 实验步骤准备实验，建立好 plate 布局，至少用 3 个复孔来测试每一个条件，用 4～6 个复孔来优化阴性对照。第 1 天
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
. This assay is highly sensitive, quantitative, easy to use, and amenable to high-throughput screening. For these reasons, the ELISPOT assay is considered by many as a gold standard for monitoring cellular immune responses. Until recently, ELISPOT assays
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