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- 2025年07月09日
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Nylon Membrane and Net FiltersNylon filters are compatible with a broad range of solvents. Two types are available: membrane filters with pore sizes ranging from 0.20 to 1.2 µm and woven net filters (NY..) with mesh openings ranging from 10 to 180 µm. |
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详细描述见链接：http://www.millipore.com/catalogue/item/NY1009000
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文献和实验Hybridization of High Density Arrayed BAC Nylon Filter Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pooled in a combined probe Isolation and purification of cDNA inserts using polymerase
1‐, 5‐, and 10‐mL plastic syringes (BD) 10‐, 25‐, 100‐, 250‐, and 500‐mL round‐bottom flasks (Kontes) Rubber septa for 14/20
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
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