- 询价
- 16-218
- 2025年12月03日
- Immunofluorescence;Western Blotting
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunofluorescence;Western Blotting
- 宿主：
Mouse
- 适应物种：
Human
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
1
- 规格：
100 μg
Product Images
IB: Acid extracts from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, WB | Mouse | Purified | Monoclonal Antibody |
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
- Immunofluorescence
- Western Blotting
- HIST3H3
- H3FT
- H3/t
- H3t
- MGC126888
- H3/g
- H3.4
- H3T
- MGC126886
Product Family Information
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
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A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806. 详细描述见链接：http://www.millipore.com/catalogue/item/16-218
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
solution contains 100 mMMgCl 2 , 1 mM un-labelled ATP, and approximately 30 MBq/mL [g- 32 P]ATP. 5. Phospho-glycogen synthase 2 (pGS2) peptide is available fromUpstate (#12-241) and the pGS2 10× stock solution contains200 μM pGS2 in kinase buffer
Proteins on Glass Slides. Proteins were dissolved in 40% glycerol, 60% PBS, pH 7.5 at a concentration of 100 μg/ml unless indicated otherwise. For Figs. 1, 5, 6, and 7, the proteins were spotted on aldehyde slides using a GMS 417 Arrayer (Affymetrix
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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