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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified Rat IgG, whole molecule
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Rat
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX02819
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Based on IEP, no cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse.
- 抗体英文名:
OneStep Polymer HRP anti-rat (Ready-to-Use)
- 抗体名:
OneStep Polymer HRP anti-rat (Ready-to-Use)
- 规格:
10 ml
Mouse Spleen sections were stained with rat anti-B220 antibody and then detected by GTX02819 OneStep Polymer HRP anti-rat (Ready-to-Use).
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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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