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文献和实验v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
over control conditioned media-treatedcells. B A monoclonal HEK293T cell line stably expressing pBARVS was generated as described in Section 3.4 . Cells weretreated with either control conditioned media or Wnt3a-conditioned media for 30 h.Control reporters
In Situ Hybridization Protocols
antibody (coupled to alkaline phosphatase) diluted to a final concentration of 1:2000 in 20% sheep serum in PBT at 4deg.C overnight. Wash three times in PBT at room temperature for 30 minutes each. Wash twice in Alkaline Phosphatase buffer
Anderson Lab In Situ Hybridization Protocols
in 4% paraformaldehyde in DEPC-PBS at room temperature for 20 minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age
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