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文献和实验a cell suspension in HBSSTransfer into Eppendorf tube:0.5 ml of 0.4% Trypan blue solution0.3 ml of HBSS0.2 ml of cell suspension in HBSS (= dilution 1 : 5)Allow to stand for 5 to 15 minutesNote: after prolonged incubation, viable cells start
Trypan blue viability test Outline: Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note : Trypan blue has a greater affinity for serum proteins than for cellular protein
Single tube confirmation PCR protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR
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