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文献和实验Purification of Plasmid from 50 ml-culture
2. Spin the bacterial culture at 6,000 rpm for 10 min at 4 C. Discard the supernatant. 3. (0ptional) Suspend the cell in about 20 ml of deionized water. Spin again. Discard the supernatant. Remove all of the supernatant fluid using pipetman.
Manufacture of Measles Viruses
amplified in Vero cells adapted to serum-free growth. Cell culture supernatant containing the measles virus is clarified by filtration to remove intact Vero cells and other debris, and then treated with Benzonase� in the presence of magnesium chloride
Detection of Measles, Mumps, and Rubella Viruses
spread and elimination. The purpose of this chapter is to provide guidance for the laboratory diagnosis of measles, mumps, and rubella virus infections. Where assays are commercially available or previously published, the appropriate references
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