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文献和实验in salt solutions containing 8 M urea, whereas the cell cytoplasm and the majority of nuclear components, except H3/H4, are washed away. This protocol yields highly purified H3/H4 in a few minutes without the use of chromatography steps. The acid
Electro-elution of nucleic acids from agarose and polyacrylamide gel slides
acid in the high-salt cushion yields material suitable for subsequent enzymatic, chemical or biological manipulations. Instructions 1. Fill with buffer making sure the clamp on the drain hose is closed, the other two clamps open. Buffer: 20mM Tris HCl
Electro-elution of nucleic acids from agarose and polyacrylamide gel slides.
within a high salt cushion resting at the bottom of the channel. Alcohol preciptiation of the nucleic acid in the high-salt cushion yields material suitable for subsequent enzymatic, chemical or biological manipulations. Instructions 1. Fill with buffer
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