BD特约代理商|352235 圆底试管（流式管）1400RC

OLIGO PURIFICATION on acrylamide gels

dried-down oligo in 40μl TE. Add 20μl formamide dye. 3. Prepare a 12% polyacrylamide gel (20:1 acrylamide:bis-acrylamide). 6.3ml 20x TBE 32ml 7M urea 38ml 40% acrylamide/7M urea 50ml dH2O 375μl ammonium persulphate 75μl TEMED 4. Cast gel in 25 x

HLA-I重链、轻链的制备（包涵体的制备）

酶 （4）1.0M  MgCl2 （5）2mg/ml DNA酶I，溶于50%甘油，75mM NaCl （6）Triton-X 100 （7）1M DTT （8）重悬液（PH8.0）50mM  Tris-HCl(PH8.0)25%(W/V) 蔗糖 1mM EDTA 0.1%(W/V) 叠氮钠 10mM DTT(新鲜加入) （9）洗涤液I(PH 8.0)50mM Tris-HCl(PH8.0)0.5% Triton-X 100 100mM NaCl 1mM

Cloning of small RNAs with 5’ phosphate and 3’ OH ends

. 12.5 ml makes two 0.75mm gels   5.25g Urea 625 µl 10X TBE 4.7 ml 40% acrylamide (19:1 acrylamide:bis-acrylamide) Add water to 12.5 ml and dissolve urea Add 87.5 µl 10% APS