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文献和实验DOP-PCR OF DNA FOR COMPARATIVE GENOMIC HYBRIDIZATION
In designing the PCR reaction, be sure to include a negative PCR control (blank without DNA), a positive control (we use MPE-600 breast tumor cell line DNA) and enough reference DNA for all of the planned CGH reactions. METHOD
volume of isopropanol and precipitate at room temperature for 5 minutes. Centrifuge at 9,000 rpm for 30 minutes in the RC5-B using the GS3 rotor. Decant the supernatant and drain the DNA pellet. 9b. Resuspend each DNA pellet in 20 ml 10:1 TE buffer, and add 40 ml
, and IMGM Laboratories, both located in Martinsried, Germany, reported using the Roche GS Junior Benchtop System to sequence clinically relevant protein coding DNA (exons) and identify genomic variations in solid tumors treated with an antibody-based
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