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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
of nonspecific interactions. Curr. Protoc. Protein Sci. 72:27.3.1?27.3.24. © 2013 by John Wiley & Sons, Inc. Keywords: protein microarray; microarray analysis; extracellular protein; multivalent binding; protein?protein interaction
(NaKC4H4O6• 4H2O)ReagentsLowry stock reagent49 ml Lowry A0.5 ml Lowry B0.5 ml Lowry CFolin's Reagent: Phenol reagent - 2N (Folin - Ciocalteau reagent).Dilute 1:1 in diH2O before use.Standard: Bovine serum albumin (BSA),lysozyme or other protein
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