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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. autoclave, cool, and add: 1x MS vitamines (SIGMA #M-7150). Take 1ml of 1000x stock prepared by disolving 10.3gr in 100ml of water. antibiotic (kanamycin 50mg/l). Top agar: 1x Murashige&Skoog salts. 1% sucrose. adjust pH
Background Required Solutions High salt buffer: PBS or TBS buffer supplemented with 0.5 M NaCl and 0.2% SDS Procedure Follow the Standard Immunodetection Method through primary and secondary antibody incubations. After the secondary
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
by fluorescence or indirectly with either anti-tetramethylrhodamine or anti�Texas Red dye antibodies (A6397, A6399; Section 7.4). MitoTracker Green FM Probe Mitochondria in cells stained with nanomolar concentrations of our patented MitoTracker Green FM dye (M
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