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文献和实验Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1 –3 ). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides
Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques (by PCR of phage insert, cycle sequencing using ABI dye terminator kits and automated sequencing on an ABI 373A) (1) Core out a single
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