Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers

Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1 –3 ). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides (antigens) are expressed as a β-galactosidase fusion protein in E. coli . Because the lacZ gene has a strong promoter, the fusion proteins comprise from 0.1–4% of the total E. coli proteins (1 –3 ). The expressed fusion antigens are transferred onto nitrocellulose filters and then probed with antibodies to identify the targets containing the specific, cognate epitope.