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文献和实验Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
Plant DNA methylation analysis by bisulfite genomic sequencing
and Wassenegger (2000). Back to top Procedure Preparation of Plant Genomic DNA Prepare plant genomic DNA using a Qiagen DNeasy Plant Maxi or Mini kit (see comment 1). Prior to the bisulfite-treatment, the plant genomic DNA is cleaved
Differential cDNA Screening Procedures
0.5 ul Incubate at 10-150C overnight continue at RT the next day. 9. Packaging follows next day. * Prepping the Lambda vector before cloning. 1. We prepare 25 ug of lambda vector. The Lambda DNA is (25ug/100 ul of O.1 X TE) heated to 68 C
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