takara特约代理商|1160A Nco1

【原创】山寨Stratagene“QuikChange Site-Directed Mutagenesis Kits”实验方案

。 7. 从每个平板上挑取6个克隆，提取质粒并进行酶切分析。鉴定无误后送交测序。 附件即为所引用的文献 实验中所用试剂： 1. PrimeSTARTM HS DNA polymerase，Takara，购自本地代理商 2. Dpn I ，Fermentas，购自上海生工 3. Cycle-pure Kit，Omega，购自本地代理商 4. 自制电感受态 5. MicroPulser Electroporation Apparatus（电转化仪），美国Bio

Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells

combinations. Two types of products are formed, crossovers (CO) and gene conversions not associated with a crossover, also called noncrossovers (NCO). They result from the repair through differentiated pathways of a common initiating event, DNA double-strand

Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen

, quantification and characterization of NCO events arising at low frequencies at recombination hotspots.