• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells

        互联网

        422
        The recombination between homologous chromosomes during the prophase of the first meiotic division plays an essential role in the formation of euploid gametes, as well as contributing to genetic diversity through the generation of new allele combinations. Two types of products are formed, crossovers (CO) and gene conversions not associated with a crossover, also called noncrossovers (NCO). They result from the repair through differentiated pathways of a common initiating event, DNA double-strand breaks, formation of which is programmed during early meiotic prophase. In contrast to CO, little is known on the frequency, distribution, and regulation of NCO in mammals, mostly due to the technical challenge represented by their detection. However, the development of batch sperm-typing methods allowed for the detection of meiotic NCO products at meiotic recombination hotspots in human and mice. Several recent studies based on this technique demonstrated that mammalian CO hotspots are sites of recombination initiation and that the formation of CO and NCO has different genetic requirements. Here, we describe a method for detecting and characterizing CO and NCO, which has been applied to the analysis of the mouse Psmb9 recombination hotspot.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序