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文献和实验In Situ Hybridization to RNA in Whole Embryos
As indicated in the preceding chapter, few techniques have had such a major impact on progress in the field of developmental biology as in situ hybridization of labeled RNA or DNA probes to detect specific mRNAs in embryonic tissues
Sequential RNA and DNA fluorescence in situ hybridization
was performed against IL-4 using a DIG-conjugated nick-translated BAC probe (middle). Nuclei were counter-stained with DAPI (blue). Back to top References Chakalova L, Carter D, Fraser P (2004) RNA fluorescence in situ hybridization tagging
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Geraldine Seydoux and Andrew Fire Adapted from Seydoux, G. and Fire, A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. In C. elegans: Modern Biological Analysis of an Organism. Methods
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